Fluorogenic assay of alkaline phosphatase activity based on the modulation of excited-state intramolecular proton transfer

Bioorganic & Medicinal Chemistry Letters
2012.0

Abstract

A new fluorogenic substrate 1, which enables the fast and quantitative analysis of alkaline phosphatase activity, has been developed. Selective enzymatic hydrolysis of 1 instantly generated fluorescent compound 2 in aqueous media, which undergo an excited-state intramolecular proton transfer process, resulting in a remarkable fluorescence turn-on signal with an unusually large Stokes shift.

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