The rhizomes of <i>Bulbine natalensis</i> furnished six previously unreported anthraquinone derivatives (<b>1</b>-<b>6</b>), together with eight known specialized metabolites. Their structures were determined by interpretation of 1D and 2D NMR and HRESIMS data. The absolute configurations of compounds <b>1</b>-<b>6</b> were determined by specific rotation and circular dichroism experiments. The isolated compounds were evaluated for antimicrobial activities, and compound <b>1</b> was found to be a moderate inhibitor (IC<sub>50</sub> 0.02 μM) against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA).