The aim of the present study was to identify potentially useful natural compounds for the development of novel therapeutic agents to inhibit metastasis. A phytochemical investigation of Pachysandra terminalis resulted in the isolation of seven new pregnane alkaloids, terminamines A−G (1−7), and seven known alkaloids (8−14). The structures of 1−7 were elucidated by 1D- and 2D-NMR spectroscopic and mass spectrometric methods. Compounds 1−5 and 8−14 inhibited the migration of MB-MDA-231 breast cancer cells induced by the chemokine epithelial growth factor. In addition, compound 1 inhibited phosphorylation of integrin β1, which plays an important role in MB-MDA-231 cell adhesion and metastasis.