The fruits of Capparis spinosa of the family Capparidaceae grow wild in different parts of Iran and are especially widely distributed in Dashte-Moghan [1]. Previous studies refer to the possible exploitation of some of the seed fats of the Capparidaceae family for industrial purposes. Only a few studies on the fatty acid composition and other characteristics of seed oil from C. spinosa are available [2–6]. This study was made to analyze the chemical properties of seeds and oil extracted from Capparis spinosa collected from different regions of Dashte-Moghan in order to determine the possibility of commercial utilization and to assess the potential use of the extracted oil in the edible oil industry.