<jats:title>Abstract</jats:title><jats:p>From a mutant of <jats:italic>Diplodia macrospora</jats:italic>, chaetoglobosin <jats:bold>M (5)</jats:bold>, ergosterol, β‐sitosterol, and a third sterol, most likely stigma‐5,7,22‐trien‐3β‐ol, were isolated. Metabolite <jats:bold>5</jats:bold> is a new member of the (<jats:italic>1H</jats:italic>‐indol‐3‐yl)‐substituted 10,11‐diethyl‐10,11‐dinorcytochalasans. Its structure was determined by <jats:sup>1</jats:sup>H‐ and <jats:sup>13</jats:sup>C‐NMR and mass spectrometry.