The fungus Curvularia pallescens DSM 62482 produces spirostaphylotrichins A, C, D, R, Q and the new spirostaphylotrichins U and V in submerged culture under limited supply of nitrogen. The configuration of spirostaphylotrichin R could be determined as the 3,4-cis-diol. For spirostaphylotrichins U and V, no phytotoxic activity could be detected.