<jats:title>Abstract</jats:title><jats:p>The isolation and structure elucidation of the new Spirostaphylotrichins N (<jats:bold>2</jats:bold>), O (<jats:bold>3</jats:bold>), P (<jats:bold>4</jats:bold>), and T (<jats:bold>7</jats:bold>) from a mutant strain of S. <jats:italic>coccosporum</jats:italic> are described. The biogenetic relationship of the known spirostaphylotrichins is discussed.