<jats:title>Abstract</jats:title><jats:p>The isolation and structure elucidation of the new spirostaphylotrichins B (<jats:bold>2</jats:bold>), C and D (<jats:bold>3/4</jats:bold>), F (<jats:bold>5</jats:bold>), Q (<jats:bold>6</jats:bold>), and R (<jats:bold>7</jats:bold>) are described. Compounds <jats:bold>2</jats:bold> and <jats:bold>3</jats:bold> are artefacts formed during the isolation of the metabolites from the cultures. The absolute configuration of spirostaphylotrichin A (<jats:bold>1</jats:bold>) has been determined by CD spectroscopy.