From the roots of Solanum abutiloides, solasodine, solafloridine and a new alkaloid have been isolated, the structure of which has been elucidated as 20-isosolafloridine [(20R, 25R)-22,26-epiminocholest-22(N)-ene-3 beta,1 diol]. The structure of an alkaloid from aerial parts of S. canense and S. fraxinifolium earlier supposed to be etioline has been recognized as 20,25-bisisoetioline [(20R,25R)-22,26-epiminocholesta-5,22(N)-diene-3 beta,16 alpha-diol].