Continuing the separation of the combined alkaloids (31.5 g) of the soluble metaphosphate part fractionated by Salzberger's method from Veratrum lobelianum, we chromatographed it on a silica gel column and separated the benzene eluate with a pH 4.0 acetate buffer, leading to the isolation of the new alkaloid germinalinine (III), C₃₉H₆₁NO₁₃, with mp 168-170°C (ether), [α]ᴰ -52.08° (pyridine). The IR spectrum of (III) showed absorption bands at 3490 cm⁻¹ (OH) and 1750, 1255 cm⁻¹ (ester C=O). Saponification of (III) gave the amino alcohol germine (IV) and l-2-methylbutyric, d-2,3-dihydroxy-2-methylbutyric, and acetic acids. Acetylation of (III) with acetic anhydride in pyridine yielded a derivative (V, mp 205-207°C) identical to germbudine triacetate (II), and methanolysis of (III) produced germbudine (I). Comparative NMR spectral analysis (19-CH₃ proton signal shifted 1 Hz downfield vs. germbudine (I)) and gentle methanolysis confirmed the acetic acid residue at C₇. Thus, germinalinine was structured as 7α-acetyl-3β-(d)-2,3-dihydroxy-2-methylbutyryl-15α-(l)-2-methylbutyrylgermine. Germbudine (for the first time) and the new alkaloid germinalinine (III) were isolated from V. lobelianum.