1. A new alkaloid, veralodinine, has been obtained by the separation of the combined alkaloids from the epigeal part of Veratrum lobelianum. 2. On the basis of the IR, UV, and NMR spectra of veralodinine and the products of its transformation, and also its conversion to known alkaloids - veralosine and tetrahydroveralodisinol - its structure and configuration has been established as 16α-acetyl-3β-D-glucopyranosido-22,26-iminocholesta-5, 22(N)-dien-24-one. © 1976 Plenum Publishing Corporation.