A number of crude amorphous alkaloidal fractions from root extracts had demonstrable anti-P-1534 leukemia activity in DBA/2 mice, and it was deemed necessary to determine whether this oncolytic activity was caused by any of the four known dimeric alkaloids or by any new entities. While the roots had been examined previously by other investigators (1), they had not been looked at utilizing the method of selective extraction, followed by column chromatography and gradient pH techniques as devised in this laboratory (2,3). The A fraction yielded two new alkaloids, vinosidine and lochnerivine, as well as mitraphylline (the oxindole of ajmalicine)—first reported as being obtained from Mitragyna macrophylla Hiern (4)—along with varying amounts of the known alkaloids ajmalicine, leurosine, virosine, perivine, VLB (obtained as both base and sulfate), leurosidine, carosidine, sitsirikine (as sulfate), and trace amounts of leurocristine (as sulfate) (5). Two other new alkaloids, leurosivine and cavincine, were obtained only as sulfates. The B fraction gave three new alkaloids, ammocalline, pericalline, and ammorosine, as well as akuammicine [first reported as being obtained from Picralima klaineana Pierre (6)] along with lochnerivine, leurosine, perivine, virosine, and lochnerine.