The continued phytochemical investigation of this pantropical plant has resulted in obtaining vinaspine and vinaphamine as free bases and vincathicine, rovidine, and desacetyl VLB as the sulfates. The total number of alkaloids obtained from this plant, utilizing selective extraction, column chromatography, and gradient pH techniques, is now 49. This investigation was pursued in an effort to elucidate the alkaloid composition of this pantropical plant as completely as possible (1). Occasionally, there is a spillover of leurocristine (and leurosidine) into the B fraction. This investigation centers on the chloroform eluate of so-called post-leurocristine B fractions. Rechromatography of the benzene-soluble material from the above fractions on deactivated alumina yielded only the known alkaloid perivine directly. Application of the gradient pH technique to each individual fraction yielded the new alkaloid vinaspine only as the base; while vincathicine, rovidine, and desacetyl VLB were obtained only as sulfates. A new alkaloid, vinaphamine, was obtained from crude amorphous material from the phosphous leurosidine-containing B fraction. These new alkaloids are listed in Table I, along with certain pertinent physical data. Their infrared spectra are reproduced separately (Figs. 1-4) as additional aids to their identification.