From Berberis boliviana, Berberis bumeliaefolia, Berberis laurina, and Berberis paucidentata, twenty-one isoquinoline alkaloids were isolated. B. laurina yielded a new compound, berberilaurine [1], a bisbenzyltetrahydroisoquinoline with two diarylether bridges in between C-7 to C-5' and C-11 to C-12'. Local pharmacopoeiae still reveal sources of new potent drugs for treatment of parasitic diseases in developing countries. In this respect, several Bolivian Indian tribes have been recorded to treat cutaneous leishmaniasis with, among others, Berberidaceous plants. In vitro, crude extracts of Bolivian barberries show leishmanicidal and trypanocidal activities. This prompted us to examine the phytochemical content of these species. The present paper describes the alkaloid content of four species collected on the Bolivian altiplano: Berberis boliviana Lechl., Berberis bumeliaefolia Schneid., Berberis laurina Billbg., and Berberis paucidentata Ruby.