<jats:title>Summary</jats:title> <jats:p>The composition of lipophilic extractives in the chloroform soluble fraction of the acetone extract from <jats:italic>Eucalyptus globulus</jats:italic> wood has been examined. The lipid extract was fractionated by solid-phase extraction on aminopropyl-phase cartridges into four different fractions of increasing polarity. The total lipid extract and the resulting fractions were analyzed by gas chromatography and gas chromatography-mass spectrometry, using high temperature capillary columns. The main compounds identified included sterols, sterol esters, fatty acids, steroid ketones, hydrocarbons and triglycerides. Minor compounds such as fatty alcohols, mono- and diglycerides, waxes and tocopherols were also identified among the lipids from <jats:italic>E. globulus</jats:italic> wood.