Polyphenolic composition (flavonoids and phenolic acids and aldehydes) is studied in the ether soluble fractions of the methanolic extracts of Eucalyptus camaldulensis, E. globulus and E. rudis wood. HPLC, TLC and GC/MS are employed for compound identification. Gallic, vanillic and ellagic acids; vanillin, syringaldehyde and sinapaldehyde; naringenin and quercetin were identified. Some flavonols, flavanones and ellagitannins were also recognized. Chemical assays for wood characterization were carried out according to Tappi Test Methods. The differences in polyphenolic composition among the three species seem not to justify their different aptitude as pulping raw material, described in literature.