Chemical investigation on the rhizome of Souliea vaginata resulted in the isolation of two new cycloartane triterpene glycosides and a new alkaloid, soulieosides D and E, and soulieotine (1-3), and their structures were elucidated on the basis of extensive NMR spectral experiments and chemical methods as (20S*,22R*,23S*,24R*)-16 beta:23;22:25-diepoxy-3 beta,24-dihydroxy-23-methoxy-9,19-cyclolanostane-3-O-beta-D-(4-acetyl)xylopyranoside (1), (20S*,22R*,23S*,24R*)-16 beta:23;22:25-diepoxy-3 beta,23,24-trihydroxy-9,19-cyclolanostane-3-O-,8-D-(3-acetyl)xylopyranoside (2), E-3-(3'-methyl-2'-butenylidene)-6-methoxy-2-indolinone (3).