In conjunction with a chemosystematic analysis of the genus Amsinckia, the novel pyrrolizidine alkaloids tessellatine and 3′,7-diacetylintermedine were isolated and structurally elucidated by chromatographic (GC and TLC) and spectroscopic (mass, and 1H and 13C NMR) experiments. The 1H and 13C NMR spectra of viridiflorine are also discussed in detail. © 1992.