Two isorhamnetin glycosides and two sulphated derivatives are reported for the first time in the genus Senecio. The flavonoids of Senecio L. species were first studied when rutin (quercetin 3-rutinoside) was reported in S. erraticus Benth. subsp. barbareifolius Koch. [1], as well as the flowers of S. jacobaea L. [2]. The 3-glucosides and 3-rutinosides of both kaempferol and quercetin, and apigenin 7-glucoside as well as the rare 3-methylquercetin were later identified in 25 Senecio species [3]. Also present was the rare 6,8-di-C-rhamnosylapigenin and, in one species only (S. tamoides), the xanthone C-glucosides, mangiferin and isomangiferin [3]. In the present study, three local species were studied. The flavonoids present were identified as the 3-glucosides of quercetin and isorhamnetin and isorhamnetin 3-rutinoside. Also present were the 3-monosulphate and 3,7(?)-disulphate of isorhamnetin (see Table 1). No C-glycosides of flavones or xanthones were detected. The presence of quercetin 3-glucoside is common in the Compositae [4,5]. On the other hand, the variation in the flavonoid chemistry within the Senecio species is not unexpected. Senecio is a cosmopolitan genus with about 1500 species, and chromosome numbers ranging from x = 5 to 90 [6]. This represents the largest variation within the tribe Senecioneae. From a recently published review on flavonoid patterns in the farinose exudate of Pityrogramma species [11], it is apparent that P. triangularis (Kaulf.) Maxon is outstanding in its farina chemistry. Only in the members of this species-complex are C-methylated flavonoids found. The following representatives have been reported so far: ceroptin, pityogrammin [2] and triangularin [3] from var. triangularis; a C-methylated dihydrochalcone from var. viscosa D. C. Eaton [4] and three C-methylated flavanones from var. pallida Weath. [5]. The farinose frond exudate of the 'ceroptin-type' of var. triangularis [6] contains a series of trace constituents, as yet unidentified. Two of these have now been isolated in small amounts [7]. One of them is a novel C-methylated flavonol, the other a novel flavone. Compound 1 appears as a dark spot on polyamide (UV 366), which turns reddish-brown after spraying with 'Naturstoffreagenz A' (b-aminodiethyl ether of diphenyl boric acid). It therefore appears to be a flavonol methylated at C-3. According to its molecular peak at m/z 312, it could be either a flavone or a 3-methylflavonol [8], but this assumption does not agree with its polarity (Rf 0.56 in solvent A). However, the 1H NMR data reveal the existence of one OMe group (δ 3.87 ppm) and two Me groups (δ 2.11, s; 2.29, s). Thus, in addition, there are two OH groups, one of which can be readily placed at C-5 (δ 12.80, broad signal). Since the 1H NMR signals, as well as characteristic MS-fragments at m/z 235 (M - 77) and 105 ('Pit C' accord. to ref. [9]), prove the presence of an unsubstituted B-ring, the second OH group must also be located on the A-ring and at C-7, although in the UV spectrum no shift is observed on addition of NaOAc.