<jats:title>Abstract</jats:title><jats:p>A glucosylceramide with novel ceramide and three novel ceramide homologs were isolated from the basidiomycete <jats:italic>Cortinarius umidicola</jats:italic> and structurally characterized. The ceramide portion of the glucocerebroside consists of a rare (4<jats:italic>E</jats:italic>,8<jats:italic>E</jats:italic>)‐9‐methyl‐4,8‐sphingadienine sphingoid base. In contrast, the three ceramide homologs, while having the same sphingoid base, contain as FA residues 2‐hydroxydocosanoic acid, 2‐hydroxytricosanoic acid, and 2‐hydroxytetracosanoic acid.