<jats:title>Abstract</jats:title><jats:p>A new 9‐methyl‐sphinga‐4,8‐dienine‐containing glucocerebroside (<jats:bold>1</jats:bold>), together with two additional known analogs, cerebrosides B and D, was isolated from the chloroform‐soluble lipid fraction of the ethanol and chloroform/methanol extract of the fruiting bodies of the basidiomycete <jats:italic>Polyporus ellisli</jats:italic> Berk. and characterized. The structure and relative stereochemistry of the new compound were identified as (2<jats:italic>S</jats:italic>,3<jats:italic>R</jats:italic>,4<jats:italic>E</jats:italic>,8<jats:italic>E</jats:italic>)‐1‐(β‐<jats:sc>d</jats:sc>‐glucopyranosyl)‐3‐hydroxy‐2‐[(<jats:italic>R</jats:italic>)‐2′‐hydroxyheptadecanoyl]amino‐9‐methyl‐4,8‐octadecadiene by means of spectroscopic (<jats:sup>1</jats:sup>H, <jats:sup>13</jats:sup>C, and two‐dimensional nuclear magnetic resonance; mass spectrometry) and chemical methods.