In the present investigation, the presence of strychnine and brucine in the seed has heen confirmed by their isolation, and their probable presence in the bark and leaf indicated by assay data. In addition, non-strychnine-brucine alkaloid fractions have been isolated from the seed and leaf. These have been tentatively called lucidine-S and lucidine-L respectively. Under the conditions of the B.P. (1932) or U.S.P. XI (1936) methods for the determination of strychnine, these fractions are estimated as strychnine, and the actual strychnine content must therefore be lower than indicated by these methods. This has been further shown by the following: the amount of strychnine isolated from a large sample of seed corresponded roughly to a 0-1 p.c. yield whereas the U.S.P. assay had indicated more than 1 p.c. of strychnine; experiments on some of the supposed strychnine residues from U.S.P. assays of the seed and leaf showed that these were approximately one-fifth as toxic as strychnine in mice. Lucidine-S and lucidine-L are amorphous, and have similar solubilities to brucine. Both were isolated in small quantities from the plant by the same method, and appear to be identical. However, neither has heen charactei'ized owing to the inahility to isolate the bases or their salts in a crystalline condition, and hence it is not known whether these alkaloid fractions are homogeneous or not.