<jats:title>Abstract</jats:title><jats:p>From the branch tissue of <jats:italic>Maytenus hookeri</jats:italic>, the endophytic strain Lz531 was isolated, and determined to belong to <jats:italic>Streptomyces</jats:italic>, according to its 16S rRNA sequence. From the extracts of the fermentation broth of <jats:italic>Streptomyces</jats:italic> sp. Lz531, two new and four known compounds were isolated. The two new compounds were identified as cyclo(<jats:sc>L</jats:sc>‐Pro‐<jats:sc>L</jats:sc>‐Val‐<jats:sc>L</jats:sc>‐Val) (<jats:bold>1</jats:bold>) and 13‐methyl‐<jats:italic>N</jats:italic>‐(2‐phenylethyl)tetradecanamide (<jats:bold>2</jats:bold>).