Buxus sempervirens L. is a decorative evergreen bush. In the present report we study the accumulation dynamics by season of total and main alkaloids from this plant cultivated in decorative settings of Tashkent. The analysis used air-dried material and alkaloids extracted by ethanol--water--glacial acetic acid (75:21:4). The ethanol was removed, the acidic solution was made basic with 10% aqueous NH3 to pH 9-10, and the alkaloids were extracted with CHCl3, then dried to produce total alkaloids. The total alkaloids were dissolved in benzene and separated by basicity using a citrate--phosphate buffer at pH 8.0-2.0 (0.5 pH intervals). Fractions at pH 8.0, 7.5, 7.0, and 6.5 were chromatographed on an aluminum oxide column (Brockman II) with elution by ether--ethanol to isolate cyclobuxine-D and cyclovirobuxine-D. Quantitative analysis showed that the total content of alkaloids increases with increasing age of the plant. The content of individual alkaloids in various plant organs varies depending on the collection time. The highest content of alkaloids is observed in first-year twigs; the lowest, in older branches. The content of main alkaloids in all organs is almost the same.