A total of 18 known carotenoids and three new carotenoids, (3S, 4R, 3%S, 6%R)-4-hydroxylutein, (3S, 3%S, 4%R)-4-keto-4%-hydroxydiatoxanthin, and (3S, 3%S, 4%R)-4-keto-4%-hydroxyalloxanthin were isolated from the goldfish Carassius auratus. On the basis of structural consideration of the isolated carotenoids and the result of our previously reported feeding experiment of lutein A and (3R, 3%R)-zeaxanthin to goldfish, the present authors proposed the possible metabolic pathways from lutein A, lutein B, (3R, 3%R)-zeaxanthin, diatoxanthin and alloxanthin to fritschiellaxanthin, a-doradexanthin, (3S, 3%S)-astaxanthin, (3S, 3%S)-7,8-didehydroastaxanthin, and (3S, 3%S)-7,8,7%,8%-tetradehydroastaxanthin, respectively.