The Amaryllidaceae alkaloids have been extensively studied due to their structural diversity, biological activities, and biosynthetic interest, but the flowers of Amaryllidaceae plants have received little attention from phytochemists. Building on prior work isolating hippeastrine N-oxide and known alkaloids from Lycoris radiata flowers, this study reports the isolation of a novel alkaloid, incartine (1), from Lycoris incarnata flowers. Incartine, a supposed biosynthetic intermediate from galanthine (2) to narcissidine (3), was isolated alongside nine known alkaloids (galanthine (2), ungiminorine (4), ungiminorine N-oxide (5), galanthamine (6), galanthamine N-oxide (7), lycoramine (8), sanguinine (9), lycorine (10), O-demethyllycoramine (11)) and two phospholipids (1-palmitoyl-2-linoleoylphosphatidylethanolamine (12) and 1-palmitoyl-2-linoleoylphosphatidylmethanol sodium salt (13)). Structural elucidation using IR, 1H-NMR, 2D-COSY, and NOE experiments revealed incartine to be galanthine α-3,3a-epoxide, marking the first isolation of this proposed epoxy intermediate.