1. The new alkaloid veralodisine has been isolated by separating the combined alkaloids from the epigeal part of Veratrum lobelianum. 2. On the basis of the results of a study of the IR, UV, NMR, and mass spectra of veralodisine and its transformation products, and also passage to the known alkaloid veralosidine, the most probable structure and configuration of veralodisine has been found to be 16α-acetyl-3β-hydroxy-22,26-iminocholesta-5,22 (N)-dien-24-one. © 1975 Plenum Publishing Corporation.