<jats:p>Four protoberberine alkaloids, palmatine (<jats:italic>Ia</jats:italic>), jatrorrhizine (<jats:italic>Ib</jats:italic>), columbamine (<jats:italic>Ic</jats:italic>) and pseudocolumbamine (<jats:italic>Id</jats:italic>), were isolated from the bark of <jats:italic>Enantia chlorantha</jats:italic> and characterized. The corresponding acetylated tetrahydro derivatives (<jats:italic>IIa-IId</jats:italic>) were also identified.