<jats:p><jats:bold>Isolation and Structure Elucidation of Ergokonin A and B; Two New Antifungal Sterol Antibiotics from <jats:italic>Trichoderma koningii</jats:italic></jats:bold><jats:p>Isolation and structure elucidation by 2D‐NMR spectroscopy of the two new antifungal sterol antibiotics ergokonin A (<jats:bold>1</jats:bold>) and B (<jats:bold>2a</jats:bold>) from <jats:italic>Trichoderma koningii</jats:italic> are described. The structures of <jats:bold>1</jats:bold> and <jats:bold>2a</jats:bold> are derived from ergosterol and characterized by an 18‐carboxy group. In addition ergokonin A (<jats:bold>1</jats:bold>) is esterified at the 3‐hydroxy group with (2<jats:italic>S</jats:italic>,3<jats:italic>S</jats:italic>)‐3‐hydroxyleucine 3‐<jats:italic>O</jats:italic>‐sulfate (<jats:bold>3c</jats:bold>). In the course of structure elucidation of the amino acid residue the stereoisomers of 3‐hydroxyleucine <jats:italic>N</jats:italic>‐sulfamate and 3‐<jats:italic>O</jats:italic>‐sulfate (<jats:bold>3b, c</jats:bold>) have been synthesized and characterized by NMR spectroscopy.