A new purine nucleoside coupled with an amino acid derivative, N2 -(1-methoxycarbonylethyl)guanosine 1, along with bcarboline and russulaceramide was isolated from the fruiting bodies of Amanita exitialis, a newly described poisonous mushroom. Its structure was elucidated by spectroscopic methods. This is the first report of naturally occurring nucleosides in which an a-amino acid derivative is bonded through its a-amino nitrogen to a nucleobase aglycone by a C–N bond. The new compound was found to be toxic in brine shrimp lethality test (BST).