Berberine, palmatine, jatrorrhizine, oxyacanthine, berbamine, reticuline, obaberine, isocorydine, thalicmidine, and the new base berberal (I) have been isolated from leaves and young shoots of Berberis heterobotrys Wolf. The structure of berberal has been established by a study of its1H and13C NMR spectra and measurement of intramolecular NOEs, and also by mass-spectrometric analysis. © 1994, Plenum Publishing Corporation. All rights reserved.