For Berberis heteropoda, the alkaloid content of its bark of stems, roots, young shoots, and leaves was studied. By separating the total alkaloids, 13 bases were isolated, among which magnoflorine, berbamunine, oblongine, reticuline, glaucine, and thalicmidine were first isolated from this plant, and l-tetrahydroberberine was first isolated from the Berberis genus. For Berberis oblonga, the dynamics of alkaloid accumulation in roots, young stems, and leaves during different growth phases was studied. The total alkaloids in roots were highest at the end of vegetation, while those in young shoots and leaves were highest during the mass flowering period. The alkaloid level in the epigeal part decreased sharply at the end of vegetation, while that in roots increased (consistent with the laws established by S. Yu. Yunusov). The main alkaloids in roots and young shoots were berberine, magnoflorine, and oxyacanthine, while in leaves the main alkaloid was glaucine.