Two new ceramides, (2S,3S,4R)-2-N-[(20 R)-20 -hydroxypentacosanoylamino]-nonacosane-1,3,4-triol (1) and (2S,3S,4R,8E)-2- N-[(20 R)-20 -hydroxytetracosanoylamino]-8-eicosylene-1,3,4-triol (2) have been isolated from the stems of Piper betle L. collected from Baoshan city of Yunnan Province in China. Their structures were determined by spectroscopic and chemical methods.