Duas novas ceramidas N-(4-hidroxifeniletil)octacosamida (1) e rel-(2S,3S,4R,16E)-2-[(2'R)- 2'-hidroxinonadecanoilamino]-heneicosadec-16-eno-1,3,4-triol (2) foram isoladas do extrato etanólico de Acnistus arborescens. As estruturas foram elucidadas por métodos espectroscópicos (experimentos de RMN 1D e 2D, EMAR com ionização por electrospray, EM e IV).Two new ceramides, N-(4-hydroxyphenethyl)octacosamide (1) and rel-(2S,3S,4R,16E)-2- [(2'R)-2'-hydroxynonadecanoylamino]-heneicosadec-16-ene-1,3,4-triol (2) were isolated from the EtOH extract of Acnistus arborescens. The structures were elucidated by spectroscopic (1D and 2D NMR experiments, HR-ESI-MS, LR-MS and IR) methods.