<jats:title>Abstract</jats:title><jats:p>Three new alkaloids, <jats:italic>N</jats:italic>‐hydroxypaxdaphnine B (<jats:bold>1</jats:bold>), 21‐<jats:italic>O</jats:italic>‐acetylpaxdaphnine B (<jats:bold>2</jats:bold>), and methyl 17‐hydroxyhomodaphniphyllate (<jats:bold>3</jats:bold>), were isolated from the fresh fruits of <jats:italic>Daphniphyllum macropodum</jats:italic>, together with six known alkaloids. Their structures were established on the basis of extensive spectroscopic and mass‐spectrometric analyses in combination with chemical transformations.