7-Hydroxypleurocorine (I), a novel spermidine alkaloid was isolated from the leaves of Pleurostylia opposita and its structure was elucidated by means of extensive ¹H and ¹³C nmr studies involving DQF COSY, HOHAHA, HMBC and HMQC experiments. The relative stereochemistry of the molecule was also probed using NOE difference spectroscopy. 7-Hydroxypleurocorine represents a new type of spermidine derivative including a pyrrolidinone.