In the present paper we report on the isolation of two new pseudobenzylisoquinoline alkaloids from Berberis Actinacantha Mart. ex Schult. (Berberidaceae). The dried and powdered roots (40 kg) were extracted successively with light petroleum ether and with ethanol by percolation. Concentration of the ethanol extracts afforded a residue which was partitioned between an aqueous solution of citric acid and chloroform. The acid solution was basified with ammonium hydroxide and the crude alkaloids extracted with chloroform. The chloroform was evaporated and the residue (43 g) was brought on a silica gel (70—230 mesh) column. Elution was performed with chloroform containing increasing percentages of methanol and the final purification by TLC on silica gel glass plates, using methanol-ethyl acetate-benzene (1:3 :6), and methanolchloroform (5 :95) as mobil phases. In this way, berbithine (1) (2.3 mg) and dihydrotoxilamine (2) (5 mg) were obtained as amorphous materials.