A new dimeric azaphenalene alkaloid, isopsylloborine A (2), has been isolated from the ladybird beetles Halyzia 16-guttata and Vibidia 12-guttata, and its structure determined by 2D NMR methods. It is a double bond isomer of the recently described psylloborine A (1), which is also present in these beetles as a minor alkaloid. (C) 1999 Published by Elsevier Science Ltd. Ail rights reserved.