Lupinus latifolius is the species of lupine implicated as being responsible for a human deformity with the alkaloid anagyrine speculated to be the teratogen. Alkaloids were extracted in the present study from L. latifolius utilizing Soxhlet extraction. This modification proved less time consuming and significantly more efficient (p < 0.01) than the original method. Capillary gas chromatographic and mass spectrometric analysis of the alkaloid extract revealed six components. Anagyrine represented 86% of the total alkaloids and 1.14% of the plant dry weight. Lupanine, aphylline, and 5,6-dehydrolupanine accounted for 6.5, 3.9, and 1.9% of the total alkaloids, respectively. Aphyllidine and 4 hydroxylupanine were also identified in small quantities.