Three new isoquinoline alkaloids, northalifoline 7-O-β-glucopyranoside (1), (R)-isococlaurine 4'-O-β-glucopyranoside (3) and (1R,3S)-1-(4-hydroxybenzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid (4) were isolated from the tubers of Stephania perriei in addition to 12 known compounds. The structure determinations were considered based on the spectroscopic evidence including 1D and 2D NMR and HR-ESI-MS experiments. © 2021 Phytochemical Society of Europe