The growing threat of proliferation of biofilm-forming hospital strains of coagulase-negative staphylococci resistant to antibiotics determines the need for an urgent search for new effective antibacterial compounds, as well as the development of methods for the combined use of traditional and alternative antibiotics. The article presents the results of a study of the combined effect of the drug ASA a new synthetic derivative of the alkaloid isoquinoline and a low-molecular-weight cationic peptide of the lantibiotic family hominin, which inhibits the development of bacterial biofilms of the clinical strain of Staphylococcus haemolyticus and its vancomycin-resistant isolates. It was found that combinations of these compounds have a synergistic effect that suppresses the formation of biofilms of both studied strains of staphylococci at reduced concentrations of these antibacterial compounds.