A new hasubanan alkaloid, hernsubanine E(1), as well as two known compounds p-hydroxybenzaldehyde(2) and(-)-syringaresinol(3) have been isolated from the whole plants of Stephania hernandifolia by various column chromatographic methods. Their structures were identified by physicochemical properties and spectral analyses. Compounds 2 and 3 were isolated from the genus of Stephania for the first time.