Investigation of the bulbs of Narcissus bicolor growing in Spain afforded three new alkaloids, bicolorine, 5,6-dihydrobicolorine and oxoassoanine-N-oxide, whose structures were determined by mass and spectral analyses. Three known alkaloids, pretazettine, 9-O-demethylhomolycorine and 3-epimacronine were also isolated. The 13C NMR data of 3-epimacronine are reported for the first time. © 1990.