A recent chemical study of the previously uninvestigated Lupinus arbustus subspecies calcaratus resulted in the isolation and characterization of the novel bipiperidyl-indole alkaloid gramodendrine. The present study describes the identification of three additional structurally diverse alkaloids from the title plant. These include the quinolizidine base lusitanine, the piperidine derivative ammodendrine, and the indole alkylamine gramine. The total alkaloid fraction as well as the first three of the above individual alkaloids were tested for gross locomotor activity and for rotorod performance in mice. Pharmacologic data indicated that gramodendrine and ammodendrine were moderately potent (300 mg/kg, ip) in reducing spontaneous motor activity (30% of control) and in causing central nervous system depression (50% of control). © 1985, American Chemical Society. All rights reserved.