The chemical structures of four new steroidal alkaloids, axillarines C [1], D [2], E [3], and F [4], from Pachysandra axillaris were elucidated as 20α-dimethylamino-3 βbenzoylamino-2β-hydroxy-5α- regnan-4β-yl acetate [1], 20α - dimethylamino-3β-benzoylamino-5α-pregnane-2 β,4β-diol diacetate [2], 20α-dimethylamino-3β-benzoylamino-5α-pregnane-2β,4β-diol [3], and 20α-dimethylamino-3β-tigloylamino-2β-hydroxy-5α-pregnan-4β-yl acetate [4]. © 1992, American Chemical Society. All rights reserved.