The chemical structures of four new steroidal alkaloids, axillarines C [1], D [2], E [3], and F [4], from Pachysandra axillaris were elucidated as 20α-dimethylamino-3β-benzoylamino-2β-hydroxy-5α-pregnan-4β-yl acetate [1], 20α-dimethylamino-3β-benzoylamino-5α-pregnane-2β,4β-diol diacetate [2], 20α-dimethylamino-3β-benzoylamino-5α-pregnane-2β,4β-diol [3], and 20α-dimethylamino-3β-tigloylamino-2β-hydroxy-5α-pregnan-4β-yl acetate [4]. Alkaloids from Pachysandra terminalis Sieb. et Zucc. (Buxaceae) have been studied by Kikuchi et al. (7). With the exception of terminaline, all these alkaloids are derivatives of 3,20α-diamino-5α-pregnane and 3,20α-diamino-5α-pregnane with oxygen at C-4 and were called pachysandra-type alkaloids (1). Recently, we have studied the pachysandra-type alkaloids isolated from Pachysandra axillaris Franch. (Buxaceae) collected in Yunnan, China (2), and reported structural elucidation of pachyaximines A and B (3), isospropachysine (4), axillaridine A (5), and pachyaxiosides A and B (6). The present paper reports the chemical structures of four new alkaloids named axillarines C [1], D [2], E [3], and F [4].