Evidence is presented for the structure of a new limonoid glycoside, 6-acetoxy-11 alpha-hydroxy-7-oxo-14 beta, 15 beta-epoxymeliacin-1,5-diene-3-O-alpha-L-rhamnopyranoside, which occurs together with salannin and meldenin in the seeds of Melia azedarach. The glycoside shows antibacterial activity against four organisms.