Some variously substituted 1-methyl-2,6-diarylpiperidin-4-one O-benzyloximes have been synthesized and their antibacterial activity against Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae and antifungal activity against Candida albicans, Cryptococcus neoformans, Rhizopus sp, Aspergillus niger and Aspergillus flavus were evaluvated. Compounds 26 and 30 exhibited potent in vitro antibacterial activity against Salmonella typhi, Escherichia coli and Klebsiella pneumoniae. While compound 28 proved to be potent against Salmonella typhi and Klebsiella pneumoniae. Like wise, compounds 26 and 28 exerted potent in vitro antifungal activity against Cryptococcus neoformans, Aspergillus niger and Aspergillus flavus whereas 30 showed very good activity than the standard drug against all the tested organisms.