Literature

Abstract

We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Omega, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Omega whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Omega.

DOI： 10.1128/aac.01173-08

Contribution of vraSR and graSR Point Mutations to Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus

Antimicrobial Agents and Chemotherapy 2009.0

Mutated Response Regulator graR Is Responsible for Phenotypic Conversion of Staphylococcus aureus from Heterogeneous Vancomycin-Intermediate Resistance to Vancomycin-Intermediate Resistance

Antimicrobial Agents and Chemotherapy 2008.0

Genomic Analysis Reveals a Point Mutation in the Two-Component Sensor Gene graS That Leads to Intermediate Vancomycin Resistance in Clinical Staphylococcus aureus

Antimicrobial Agents and Chemotherapy 2008.0

Selection of Heterogeneous Vancomycin-Intermediate Staphylococcus aureus by Imipenem

Antimicrobial Agents and Chemotherapy 2009.0

Interaction of the GraRS Two-Component System with the VraFG ABC Transporter To Support Vancomycin-Intermediate Resistance in Staphylococcus aureus

Antimicrobial Agents and Chemotherapy 2007.0

VanA-Type Vancomycin-ResistantStaphylococcus aureus