The Pathogen Box contains 400 diverse, drug-like molecules active against neglected diseases of interest. This dataset contains biological activity confirmation data performed in the following disease areas: Tuberculosis, Malaria, Chagas disease, Leishmaniasis, Human African Trypanosomiasis, Cryptosporidiosis, Lymphatic Filariasis, Onchocerciasis, Schistosomiasis, Dengue, Chikungunya, Toxoplasmosis. For more information about the pathogen box, visit: https://www.pathogenbox.org